【文獻標(biāo)題】Effect of CCNB1 silencing on cell cycle, senescence, and apoptosis through the p53 signaling pathway in pancreatic cancer

【作者】Hui Zhang,Xuan Zhang,Xun Li,et.al

【作者單位】蘭州大學(xué)第一醫(yī)院（ First Hospital of Lanzhou University）

【文獻中引用產(chǎn)品】

人免疫球蛋白G(IgG)ELISA試劑盒

【關(guān)鍵詞】apoptosis, CCNB1, cell cycle, gene silencing, p53 signaling pathway, pancreatic cancer, senescence

【DOI】 10.1002/jcp.26816

【影響因子(IF)】6.5

【出版期刊】《Journal of Cellular Physiology》

【產(chǎn)品原文引用】

Immunohistochemistry for CCNB1‐positive expression rate

The PC and normal pancreas tissues were fixed with formaldehyde, embedded in paraffin, and cut into 4‐μm serial sections. The sections were baked in a 60℃ incubator for 1 hr, conventionally dewaxed with xylene, dehydrated with gradient ethanol and then immersed in 3% H2O2 for 10 min. The sections were subsequently washed with distilled water, placed at a high pressure for 90 s for antigen retrieval, cooled at room temperature and washed with phosphate‐buffered saline (PBS). After that, the sections were further blocked with 5% bovine serum albumin and incubated at 37℃ for 30 min. The sections were added with diluted primary antibody rabbit anti‐human CCNB1 (1:500, EPR17060; Abcam Inc., Cambridge, MA) for incubation at 4℃ overnight, followed by PBS washing. Then, the sections were added with the diluted biotin‐labeled immunoglobulin G (IgG) secondary antibody (1:100; HY90046; Shanghai HengYuan Biological Technology Co. Ltd., Shanghai, China) for incubation at 37℃ for 30 min, followed by PBS washing. 

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